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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
23/01/2020 |
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Actualizado : |
23/01/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CABRERA, A.; FRESIA, P.; BERNÁ, L.; SILVEIRA, C.S.; MACÍAS-RIOSECO, M.; AREVALO, A.P.; CRISPO, M.; PRITSCH, O.; RIET-CORREA, F.; GIANNITTI, F.; GIANNITTI, F.; FRANCIA, M.E.; ROBELLO, C. |
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Afiliación : |
ANDRÉS CABRERA, Laboratory of Host Pathogen Interactions-UBM, Institut Pasteur de Montevideo, Uruguay; PABLO FRESIA, Bioinformatics Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay; LUCÍA BERNÁ, Laboratory of Host Pathogen Interactions-UBM, Institut Pasteur de Montevideo, Montevideo, Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA PAULA AREVALO, Transgenic and Experimental Animal Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay; MARTINA CRISPO, Transgenic and Experimental Animal Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay; OTTO PRITSCH, Immunovirology Unit, Institut Pasteur de Montevideo, Montevideo Uruguay; Departamento de Inmunobiología, Facultad de Medicina, Universidad de la República, Montevideo Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul MN USA; MARIA E. FRANCIA, Laboratory of Host Pathogen Interactions-UBM, Institut Pasteur Montevideo, Uruguay; Laboratory of Apicomplexan Biology, Institut Pasteur Montevideo, Uruguay;Dpto. Parasitologia y Micologia, Facultad de Medicina, Universidad de la República, Uruguay; CARLOS ROBELLO, Laboratory of Host Pathogen Interactions-UBM, Institut Pasteur de Montevideo, Montevideo Uruguay; Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo Uruguay. |
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Título : |
Isolation and molecular characterization of four novel Neospora caninum strains. |
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Complemento del título : |
Genetics, Evolution, and Phylogeny - Short Communication. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Parasitology Research, 1 December 2019, Volume 118, Issue 12, Pages 3535-3542. Doi: 10.1007/s00436-019-06474-9 |
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ISSN : |
0932-0113 |
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DOI : |
10.1007/s00436-019-06474-9 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 11 April 2019 / Accepted: 24 September 2019 / Published online: 7 November 2019.
Funding Sponsor: Agencia Nacional de Investigación e Innovación (ANII).
Funding Text: This project was funded by grant FSSA_X_2014_1_106026 from the Uruguayan National Agency for Research and Innovation (ANII). A.C., C.S., and M.M.R. are supported by doctoral fellowships from ANII. M.E.F. is supported by a Calmette & Yersin fellowship from the Institut Pasteur International Network (RIIP). M.C., L.B., P.F., F.G., F.R.-C., O.P., M.E.F., and C.R. are researchers from the Sistema Nacional de Investigadores (SNI). |
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Contenido : |
ABSTRACT.
Neospora caninum causes neosporosis, a leading cause of bovine abortion worldwide. Uruguay is a developing economy in South America that produces milk to feed seven times its population annually. Naturally, dairy production is paramount to the country?s economy, and bovine reproductive failure impacts it profoundly. Recent studies demonstrated that the vast majority of infectious abortions in dairy cows are caused by N. caninum. To delve into the local situation and contextualize it within the international standing, we set out to characterize the Uruguayan N. caninum strains. For this, we isolated four distinct strains and determined by microsatellite typing that these represent three unique genetic lineages, distinct from those reported previously in the region or elsewhere. An unbiased analysis of the current worldwide genetic diversity of N. caninum strains known, whereby six typing clusters can be resolved, revealed that three of the four Uruguayan strains group closely with regional strains from Argentina and Brazil. The remaining strain groups in an unrelated genetic cluster, suggesting multiple origins of the local strains. Microsatellite typing of N. caninum DNA from fetuses opportunistically collected from local dairy farms correlated more often with one of the isolates. Overall, our results contribute to further understanding of genetic diversity among strains of N. caninum both regionally and worldwide.
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature. MenosABSTRACT.
Neospora caninum causes neosporosis, a leading cause of bovine abortion worldwide. Uruguay is a developing economy in South America that produces milk to feed seven times its population annually. Naturally, dairy production is paramount to the country?s economy, and bovine reproductive failure impacts it profoundly. Recent studies demonstrated that the vast majority of infectious abortions in dairy cows are caused by N. caninum. To delve into the local situation and contextualize it within the international standing, we set out to characterize the Uruguayan N. caninum strains. For this, we isolated four distinct strains and determined by microsatellite typing that these represent three unique genetic lineages, distinct from those reported previously in the region or elsewhere. An unbiased analysis of the current worldwide genetic diversity of N. caninum strains known, whereby six typing clusters can be resolved, revealed that three of the four Uruguayan strains group closely with regional strains from Argentina and Brazil. The remaining strain groups in an unrelated genetic cluster, suggesting multiple origins of the local strains. Microsatellite typing of N. caninum DNA from fetuses opportunistically collected from local dairy farms correlated more often with one of the isolates. Overall, our results contribute to further understanding of genetic diversity among strains of N. caninum both regionally and worldwide.
© 2019, Springer-Verlag GmbH Germany, part of Spr... Presentar Todo |
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Palabras claves : |
Animal health; Apicomplexa; Bovine abortion; Genetic diversity; Microsatellite; Neospora; PLATAFORMA SALUD ANIMAL. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 03240naa a2200385 a 4500
001 1060674
005 2020-01-23
008 2019 bl uuuu u00u1 u #d
022 $a0932-0113
024 7 $a10.1007/s00436-019-06474-9$2DOI
100 1 $aCABRERA, A.
245 $aIsolation and molecular characterization of four novel Neospora caninum strains.$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 11 April 2019 / Accepted: 24 September 2019 / Published online: 7 November 2019. Funding Sponsor: Agencia Nacional de Investigación e Innovación (ANII). Funding Text: This project was funded by grant FSSA_X_2014_1_106026 from the Uruguayan National Agency for Research and Innovation (ANII). A.C., C.S., and M.M.R. are supported by doctoral fellowships from ANII. M.E.F. is supported by a Calmette & Yersin fellowship from the Institut Pasteur International Network (RIIP). M.C., L.B., P.F., F.G., F.R.-C., O.P., M.E.F., and C.R. are researchers from the Sistema Nacional de Investigadores (SNI).
520 $aABSTRACT. Neospora caninum causes neosporosis, a leading cause of bovine abortion worldwide. Uruguay is a developing economy in South America that produces milk to feed seven times its population annually. Naturally, dairy production is paramount to the country?s economy, and bovine reproductive failure impacts it profoundly. Recent studies demonstrated that the vast majority of infectious abortions in dairy cows are caused by N. caninum. To delve into the local situation and contextualize it within the international standing, we set out to characterize the Uruguayan N. caninum strains. For this, we isolated four distinct strains and determined by microsatellite typing that these represent three unique genetic lineages, distinct from those reported previously in the region or elsewhere. An unbiased analysis of the current worldwide genetic diversity of N. caninum strains known, whereby six typing clusters can be resolved, revealed that three of the four Uruguayan strains group closely with regional strains from Argentina and Brazil. The remaining strain groups in an unrelated genetic cluster, suggesting multiple origins of the local strains. Microsatellite typing of N. caninum DNA from fetuses opportunistically collected from local dairy farms correlated more often with one of the isolates. Overall, our results contribute to further understanding of genetic diversity among strains of N. caninum both regionally and worldwide. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
653 $aAnimal health
653 $aApicomplexa
653 $aBovine abortion
653 $aGenetic diversity
653 $aMicrosatellite
653 $aNeospora
653 $aPLATAFORMA SALUD ANIMAL
700 1 $aFRESIA, P.
700 1 $aBERNÁ, L.
700 1 $aSILVEIRA, C.S.
700 1 $aMACÍAS-RIOSECO, M.
700 1 $aAREVALO, A.P.
700 1 $aCRISPO, M.
700 1 $aPRITSCH, O.
700 1 $aRIET-CORREA, F.
700 1 $aGIANNITTI, F.
700 1 $aGIANNITTI, F.
700 1 $aFRANCIA, M.E.
700 1 $aROBELLO, C.
773 $tParasitology Research, 1 December 2019, Volume 118, Issue 12, Pages 3535-3542. Doi: 10.1007/s00436-019-06474-9
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
12/09/2014 |
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Actualizado : |
08/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 2 |
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Autor : |
FITZGERALD, M.A.; BERGMAN, C.J.; RESURRECCION, A.P.; MOLLER, J.; JIMENEZ, R.; REINKE, R.F.; MARTIN, M.; BLANCO, P.; MOLINA, F.; CHEN, M.-H.; KURI, V.; ROMERO, M.V.; HABIBI, F.; UMEMOTO, T.; JONGDEE, S.; GRATEROL, E.; REDDY, K.R.; BASSINELLO, P.Z.; SIVAKAMI, R.; RANI, N.S.; DAS, S.; WANG, Y.J.; INDRASARI, S.D.; RAMLI, A.; AHMAD, R.; DIPTI, S.S.; XIE, L.; LANG, N.T.; SINGH, P.; PORO, D.C.; TAVASOLI, F.; MESTRES, C. |
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Afiliación : |
BLANCO BARRAL, PEDRO HORACIO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MOLINA CASELLA, FEDERICO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Addressing the dilemmas of measuring amylose in rice. |
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Fecha de publicación : |
2009 |
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Fuente / Imprenta : |
Cereal Chemistry, 2009, v. 86, no. 5, p. 492-498. |
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ISSN : |
0009-0352 |
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DOI : |
10.1094/CCHEM-86-5-0492 |
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Idioma : |
Inglés |
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Contenido : |
Amylose content is a parameter that correlates with the cooking behavior of rice. It is measured at the earliest possible stages of rice improvement programs to enable breeders to build the foundations of appropriate grain quality during cultivar development. Amylose is usually quantified by absorbance of the amylose-iodine complex. The International Network for Quality Rice (INQR) conducted a survey to determine ways that amylose is measured, reproducibility between laboratories, and sources of variation. Each laboratory measured the amylose content of a set of 17 cultivars of rice. The study shows that five different versions of the iodine binding method are in use. The data show that repeatability was high within laboratories but reproducibility between laboratories was low. The major sources of variability were the way the standard curve was constructed and the iodine binding capacity of the potato amylose used to produce the standard. Reproducibility is much lower between laboratories using a standard curve of potato amylose alone compared with those using calibrated rice cultivars. This study highlights the need to standardize the way amylose is measured, and presents research avenues for doing so. |
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Thesagro : |
AMILOSA; ARROZ; CALIDAD CULINARIA. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02591naa a2200553 a 4500
001 1050218
005 2019-10-08
008 2009 bl uuuu u00u1 u #d
022 $a0009-0352
024 7 $a10.1094/CCHEM-86-5-0492$2DOI
100 1 $aFITZGERALD, M.A.
245 $aAddressing the dilemmas of measuring amylose in rice.$h[electronic resource]
260 $c2009
520 $aAmylose content is a parameter that correlates with the cooking behavior of rice. It is measured at the earliest possible stages of rice improvement programs to enable breeders to build the foundations of appropriate grain quality during cultivar development. Amylose is usually quantified by absorbance of the amylose-iodine complex. The International Network for Quality Rice (INQR) conducted a survey to determine ways that amylose is measured, reproducibility between laboratories, and sources of variation. Each laboratory measured the amylose content of a set of 17 cultivars of rice. The study shows that five different versions of the iodine binding method are in use. The data show that repeatability was high within laboratories but reproducibility between laboratories was low. The major sources of variability were the way the standard curve was constructed and the iodine binding capacity of the potato amylose used to produce the standard. Reproducibility is much lower between laboratories using a standard curve of potato amylose alone compared with those using calibrated rice cultivars. This study highlights the need to standardize the way amylose is measured, and presents research avenues for doing so.
650 $aAMILOSA
650 $aARROZ
650 $aCALIDAD CULINARIA
700 1 $aBERGMAN, C.J.
700 1 $aRESURRECCION, A.P.
700 1 $aMOLLER, J.
700 1 $aJIMENEZ, R.
700 1 $aREINKE, R.F.
700 1 $aMARTIN, M.
700 1 $aBLANCO, P.
700 1 $aMOLINA, F.
700 1 $aCHEN, M.-H.
700 1 $aKURI, V.
700 1 $aROMERO, M.V.
700 1 $aHABIBI, F.
700 1 $aUMEMOTO, T.
700 1 $aJONGDEE, S.
700 1 $aGRATEROL, E.
700 1 $aREDDY, K.R.
700 1 $aBASSINELLO, P.Z.
700 1 $aSIVAKAMI, R.
700 1 $aRANI, N.S.
700 1 $aDAS, S.
700 1 $aWANG, Y.J.
700 1 $aINDRASARI, S.D.
700 1 $aRAMLI, A.
700 1 $aAHMAD, R.
700 1 $aDIPTI, S.S.
700 1 $aXIE, L.
700 1 $aLANG, N.T.
700 1 $aSINGH, P.
700 1 $aPORO, D.C.
700 1 $aTAVASOLI, F.
700 1 $aMESTRES, C.
773 $tCereal Chemistry, 2009$gv. 86, no. 5, p. 492-498.
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